King Fahd Medical Research Center

KFMRC

Molecular Cancer Biology and Therapy Researches

The lab diagnoses and distinguishes tumors as a state of genetic instability and genetic sequential creation that changes the functions of many different molecules and networks within a single cell as well as changes in interaction with the surrounding environment. The application of biology to cancer systems is based on the use of computer and mathematical methods and means to decode complex tumors in addition to cancer mismatches. Cancer research has identified dangerous cancer genes or inactive tumor genes in cancer causes.

Research Areas

The Research Lab is concerned with conducting research in the following areas

Tumor Development

Identify the functional SNPs and transcript variants and understanding the transcription mechanism that regulate tumor development and metastasis in Cancer with especial references to Ovarian and Colorectal Cancer.

Novel Biomarker,

Whole transcriptome analysis studies to identify novel biomarker, potential drug target in Ovarian and Colorectal Cancer.

SNPs

tudy the functional SNPs and their effect on the Multi-Drug Resistance Genes (MDR) expression in order to establish correlation with chemotherapeutic response outcome in ovarian cancer patients.

Epigenetic

Identifying the functional contribution of epigenetic in cancer development and treatment response and improve the epigenetic anticancer drug delivery using nanoparticles encapsulation.

Services

The laboratory provides a number of diverse services as well as training and assistance to bachelor's and graduate students as follows:

Cycle Sequencing & Analysis

Cycle & Automated DNA sequencing, western blot analysis, Single Cell Next Generation Sequencing & data analysis

Genomic

Genomic Deoxyribonucleic acid(DNA) extraction, Ribonucleic acid(RNA) isolation

Cell culture

Process by which real nucleus and primitive nucleus are developed under controlling conditions

PCR

Polymerase Chain Reaction amplification, one step and two step Real Time PCR (RT-PCR)

Publications

Recent Publications of Molecular Cancer Biology and Therapy Research Lab

Absarul Haque, Khalid Hussain Wali Sait, Qamre Alam, Mohammad Zubair Alam, Nisreen Anfinan, Abdul Wahab Noor Wali and Mahmood Rasool (2020). MDR1 Gene Polymorphisms and Its Association with Expression as a Clinical Relevance in Terms of Response to Chemotherapy and Prognosis in Ovarian Cancer. Front. Genet; 2020 | https://doi.org/10.3389/fgene.2020.00516. Link: https://pubmed.ncbi.nlm.nih.gov/32528530/
Beg MA, Nieschlag E, Abdel-Meguid TA, Alam Q, Abdelsalam A, Haque A, Mosli HA, Bajouh OS, Abuzenadah AM, Al-Qahtani M (2019). Genetic investigations on causes of male infertility in Western Saudi Arabia. Andrologia. 2019 Jul; 51(6):e13272. Link: https://pubmed.ncbi.nlm.nih.gov/30907014/
Rasool M, Malik A, Ashraf M A B, Arooj M, Kiran A, Waquar S, Ayyaz U, Zahid A, Zaheer A, Jabbar A, Zain M, Raza A, Mehmood A, Qaisrani T B, Mirza Z, Al-Qahtani M H, Karim S, Haque A (20018). Role of diagnostic factors associated with antioxidative status and expression of matrix metalloproteinases (MMPs) in patients with cancer therapy induced ocular disorders. Saudi J Biol Sci 25(8): 1724–1728. Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303172/
Rasool M, Malik A, Butt T T, Ashraf M A B, Rasool R, Zahid A, Waquar S, Asif M, Zaheer A, Jabbar A, Zain M, Mehmood A, Qaisrani T B, Malik I R, Khan S U, Mirza Z, Haque A, Al-Qahtani M H, Karim S (2019). Implications of advanced oxidation protein products (AOPPs), advanced glycation end products (AGEs) and other biomarkers in the development of cardiovascular diseases. Saudi J Biol Sci. 26(2): 334–339. Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6717110/
Haque A, Alam Q, Alam MZ, Azhar EI, Sait KH, Anfinan N, Mushtaq G, Kamal MA, Rasool M (2016). Current Understanding of HSP90 as a Novel Therapeutic Target: An Emerging Approach for the Treatment of Cancer. Curr Pharm Des. 22(20): 2947-59. Link: https://pubmed.ncbi.nlm.nih.gov/27013225/

Hafeez S, Urooj M, Saleem S, Gillani Z, Shaheen S, Qazi MH, Naseer MI, Iqbal Z, Ansari SA, Haque A, Asif M, Mir MA, Ali A, Pushparaj PN, Jamal MS, Rasool M(2016). BAD, a Proapoptotic Protein, Escapes ERK/RSK Phosphorylation in Deguelin and siRNA-Treated HeLa Cells. PLoS One. 8;11(1). Link: https://pubmed.ncbi.nlm.nih.gov/26745145/
Jabir NR, Firoz CK, Kamal MA, Damanhouri GA, Alama MN, Alam Q, Haque A, Almehdar HA, Tabrez S(2017). Assessment of IL-18 Serum Level and Its Promoter Polymorphisms in the Saudi Coronary Artery Disease (CAD) Patients. J Cell Biochem.118 (7):1849-1854. Link: https://pubmed.ncbi.nlm.nih.gov/28059484/
Rasool M, Malik A, Hussain M, Haq KA, Butt K, Basit A, Naseer MI, Asif M, Shaikh R, Mustafa MZ, Alam Q, Rasool G, Ahmad W, Haque A, Kamal MA(2017). DARPins Bioengineering and its Theranostic Approaches: Emerging Trends in Protein Engineering. Curr Pharm Des. 23(11):1610-1615. doi: 10.2174/1381612822666161208121829. Link: https://pubmed.ncbi.nlm.nih.gov/27928961/
Mohamed AB, Hindawi SI, Al-Harthi S, Alam Q, Alam MZ, Haque A, Ahmad W, Damanhouri GA(2016). Allelic variance among ABO blood group genotypes in a population from the western region of Saudi Arabia. Blood Res. 51(4):274-278. doi: 10.5045/br.2016.51.4.274. Link: https://europepmc.org/article/PMC/5234243
Alshawli, AS, Wurdak, H, Wood, IC, Ladbury, JE. Histone deacetylase inhibitors induce medulloblastoma cell death independent of HDACs recruited in REST repression complexes. Mol Genet Genomic Med. 2020; 00:e1429. Link: https://onlinelibrary.wiley.com/doi/full/10.1002/mgg3.1429