Main Page
About KFMRC
Mission and Vision
Hierarchical Structure
Research
Researchers
Publications
Research Units
Academics
Facilities
Support Units
Core Labs
Animal House
News
عربي
English
About
Admission
Academic
Research and Innovations
University Life
E-Services
Search
King Fahd Medical Research Center
Document Details
Document Type
:
Article In Journal
Document Title
:
Inactive matrix metalloproteinase 2 is a normal constituent of human glomerular basement membrane. An immuno-electron microscopic study
Inactive matrix metalloproteinase 2 is a normal constituent of human glomerular basement membrane. An immuno-electron microscopic study
Document Language
:
English
Abstract
:
Remodelling of the extracellular matrix requires tight control not only of matrix synthesis, but also of matrix degradation. Control of matrix degradation is achieved mainly through the matrix metalloproteinase (MMP) enzymes. In the glomerulus, MMP-2 and MMP-9 are believed to be particularly important, as they have activity against type IV collagen. This study has demonstrated by immuno-electron microscopy that most of the immunoreactivity for MMP-2 in the normal glomerulus is located within the glomerular basement membranes and mesangial matrix. mRNA for MMP-2 is also detectable in normal glomeruli, but the other main gelatinase, MMP-9, could not be localized by immuno-electron microscopy. In the normal glomerulus, it seemed likely that MMP-2 is present in an inactive form. To confirm this, in situ zymography was carried out using frozen sections of normal kidney. Baseline activity of normal kidney was relatively weak, but this was dramatically increased by chemical activation of metalloproteinases. The results imply that MMP-2, in an inactive form, is a normal constituent of the extracellular matrix and glomerular basement membranes. Activation would presumably render the matrix 'self-degrading'; membrane-bound MMPs (MT-MMPs) seem particularly likely to be involved in leukocyte penetration of basement membranes in inflammation
ISSN
:
1096-9896
Journal Name
:
The Journal of pathology
Volume
:
191
Issue Number
:
1
Publishing Year
:
1421 AH
2000 AD
Article Type
:
Article
Added Date
:
Monday, July 5, 2010
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
سوسن جلله
Jalalah, SM
Investigator
Doctorate
بيتر فورنيس
Furness, PN
Researcher
Doctorate
Files
File Name
Type
Description
27332.pdf
pdf
Back To Researches Page